Pat Brown’s Lab Department of Biochemistry, Stanford University School of Medicine Howard Hughes Medical Institutehttp://biochemistry.stanford.edu/http://med.stanford.edu/http://www.hhmi.orgshapeimage_1_link_0shapeimage_1_link_1shapeimage_1_link_2
 


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DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein tumor stroma translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics rbp rbp rbp rbp rbp rbp rbp rbp microbial ecology microbial ecology microbial ecology plos plos plos plos plos plos plos plos  pat brown lab pat brown lab microenvironment microenvironment gene expression open access open access DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein tumor stroma translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics rbp rbp rbp rbp rbp rbp rbp rbp microbial ecology microbial ecology microbial ecology plos plos plos plos plos plos plos plos  pat brown lab pat brown lab microenvironment microenvironment gene expression open access open access DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein tumor stroma translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics rbp rbp rbp rbp rbp rbp rbp rbp microbial ecology microbial ecology microbial ecology plos plos plos plos plos plos plos plos  pat brown lab pat brown lab microenvironment microenvironment gene expression open access open access DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein tumor stroma translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics rbp rbp rbp rbp rbp rbp rbp rbp microbial ecology microbial ecology microbial ecology plos plos plos plos plos plos plos plos  pat brown lab pat brown lab microenvironment microenvironment gene expression open access open access DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein tumor stroma translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics rbp rbp rbp rbp rbp rbp rbp rbp microbial ecology microbial ecology microbial ecology plos plos plos plos plos plos plos plos  pat brown lab pat brown lab microenvironment microenvironment gene expression open access open access NA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein tumor stroma translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics rbp rbp rbp rbp rbp rbp rbp rbp microbial ecology microbial ecology microbial ecology plos plos plos plos plos plos plos plos  pat brown lab pat brown lab microenvironment microenvironment gene expression open access open access DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein tumor stroma translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics rbp rbp rbp rbp rbp rbp rbp rbp microbial ecology microbial ecology microbial ecology plos plos plos plos plos plos plos plos  pat brown lab pat brown lab microenvironment microenvironment gene expression open access open access DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein tumor stroma translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics rbp rbp rbp rbp rbp rbp rbp rbp microbial ecology microbial ecology microbial ecology plos plos plos plos plos plos plos plos  pat brown lab pat brown lab microenvironment microenvironment gene expression open access open access DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein tumor stroma translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics rbp rbp rbp rbp rbp rbp rbp rbp microbial ecology microbial ecology microbial ecology plos plos plos plos plos plos plos plos  pat brown lab pat brown lab microenvironment microenvironment gene expression open access open access DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein tumor stroma translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics DNA microarray genomics RNA binding protein translation microarray genomics rbp rbp rbp rbp rbp rbp rbp rbp microbial ecology microbial ecology microbial ecology plos plos plos plos plos plos plos plos  pat brown lab pat brown lab microenvironment microenvironment gene expression open

 

Each cell in our bodies expresses a specific set of genes according to a precisely controlled genetic script that gives that cell its distinctive design and capabilities. The gene expression program that unfolds during a developmental or physiological or pathological process can be read as a kind of a script for that process.

The Brown Lab investigates the gene expression scripts of the yeast and human genomes - their organization, physiological logic and the molecular mechanisms that control them.  Over the past decade, we have characterized gene expression patterns in thousands of human cells and tissues under diverse conditions. These studies have given us detailed molecular pictures of the programmed responses of the human genome to diverse physiological and pathological conditions, insights into the mechanisms by which these processes are deranged in cancer and other diseases, and new ways to diagnose, classify and predict behavior of human cancers.

Our recent work has convinced us that post-transcriptional control of the localization, translation and degradation of each messenger RNA by specific RNA-binding proteins plays a much richer and more important role in biological regulation than previously suspected.  We are now developing and using genetic, biochemical and computational approaches to systematically map out the regulatory circuitry and molecular mechanisms that control the life history of each gene’s RNA transcripts.


Most of our current efforts are focused in five areas:

  1. •Systematic investigation of the global program and molecular mechanisms of post-transcriptional regulation of gene expression in yeast and in human development, physiology and disease.

  2. •Mechanisms and logic of subcellular localization and trafficking of mRNA.

  3. •Defining the cellular and molecular architecture of human tissue and tumor microenvironments; understanding how tissues develop and maintain their precise architecture and functional specialization; understanding how tissue microenvironments influence the survival, proliferation, differentiation and physiology of normal and cancer cells.

  4. •Identifying patterns of gene expression that can be used to detect and precisely identify human cancers and predict their potential for progression or response to specific therapies.

  5. •Developing new strategies and technologies for diagnosis and early detection of cancer.


We also have a continuing interest in:

  1. •The microbial communities that live in and on the human body; what determines the distinctive composition of these communities in each individual and how they influence our physiology and pathophysiology.

  2. •The diversity and local specialization of the “stromal” cells that comprise the infrastructure of every tissue and organ and their roles in development, self-renewal and disease.

  3. •Reinventing scientific communication.

  4. •Drastically reducing the global footprint of animal farming.

  5. •Who knows what will be next?...

 

RESEARCH INTERESTS

Recent Publications: Three-dimensional tracking of single mRNA particles in S. cerevisiae using a Double-Helix Point Spread Function. Proteome-Wide Search Reveals Unexpected RNA-Binding Proteins in Saccharomyces cerevisiae. Concordant regulation of translation and mRNA decay for hundreds of targets of a human microRNA. The preclinical natural history of serous ovarian cancer: defining the target for early detection. Diverse RNA-Binding Proteins Interact with Functionally Related Sets of RNAs, Suggesting an Extensive Regulatory System Systematic identification of mRNAs recruited to argonaute 2 by specific microRNAs and corresponding changes in transcript abundance Transcriptional program induced by Wnt protein in human fibroblasts suggests mechanisms for cell cooperativity in defining tissue microenvironments. More....http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2964242/?tool=pubmedhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2964242/?tool=pubmedhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0012671http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0012671http://www.plosbiology.org/article/info%3Adoi%2F10.1371%2Fjournal.pbio.1000238http://www.plosbiology.org/article/info%3Adoi%2F10.1371%2Fjournal.pbio.1000238http://www.google.com/ighttp://www.google.com/ighttp://biology.plosjournals.org/perlserv/?request=get-document&doi=10.1371/journal.pbio.0060255http://biology.plosjournals.org/perlserv/?request=get-document&doi=10.1371/journal.pbio.0060255http://biology.plosjournals.org/perlserv/?request=get-document&doi=10.1371/journal.pbio.0060255http://www.plosone.org/article/info:doi/10.1371/journal.pone.0002126http://www.plosone.org/article/info:doi/10.1371/journal.pone.0002126http://www.plosone.org/article/info:doi/10.1371/journal.pone.0002126http://www.plosone.org/article/info:doi/10.1371/journal.pone.0000945http://www.plosone.org/article/info:doi/10.1371/journal.pone.0000945http://www.plosone.org/article/info:doi/10.1371/journal.pone.0000945Papers.htmlshapeimage_3_link_0shapeimage_3_link_1shapeimage_3_link_2shapeimage_3_link_3shapeimage_3_link_4shapeimage_3_link_5shapeimage_3_link_6shapeimage_3_link_7shapeimage_3_link_8shapeimage_3_link_9shapeimage_3_link_10shapeimage_3_link_11shapeimage_3_link_12shapeimage_3_link_13shapeimage_3_link_14shapeimage_3_link_15shapeimage_3_link_16shapeimage_3_link_17
 
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